Bacteriophage EFC1 infecting Enterococcus faecalis from chicken
The world of phage research is constantly evolving and expanding as new viruses are discovered.
One of these viruses is EFC1, a lytic phage isolated from the wastewater of a farm in Handan, China. What makes EFC1 unique is its ability to infect 87.5% of laboratory-preserved Enterococcus faecalis strains derived from chickens. A more detailed analysis of its genome revealed that it contains 89 protein-coding genes as well as tRNA molecules involved in various functions, including intron structure, DNA replication, and cell lysis.
Comparative analysis also assigned EFC1 to a new genus – Saphexavirus – within the family Siphoviridae. This discovery contributes to our understanding of phage genetics and highlights the diversity of these viruses in different environments.
Future research on EFC1 and other newly discovered phages could lead to potential applications in areas such as medicine and agriculture.
These findings are presented by the authors Qi Wang, and Na Liu in their article: Complete genome analysis of bacteriophage EFC1 infecting Enterococcus faecalis from chicken, as published on 22 June 2022.
- A novel bacteriophage, EFC1, was isolated from sewage on a farm in Handan, China.
- EFC1 can infect 87.5% of chicken-derived Enterococcus faecalis strains preserved in laboratory conditions.
- The genome of phage EFC1 consists of 56,099 base pairs and 89 predicted protein-coding genes.
- Phage EFC1 is classified as a new genus Saphexavirus phage in the Siphoviridae family.
A novel lytic Enterococcus faecalis phage, EFC1, was isolated from the sewage of a farm in Handan, China, and its genome was analyzed and described.
The phage could infect 87.5% of the chicken-derived Enterococcus faecalis preserved in our laboratory.
The genome of phage EFC1 consists of a circular double-stranded DNA with a length of 56,099 bp and a G + C content of 39.96%, containing 89 predicted protein-coding genes as well as 2 tRNAs, which are involved in phage intron, structure, transcription, packaging, DNA replication, modification, cell lysis, and other functions, indicating the genetic and functional characteristics of this phage.
Genome comparison analysis revealed that phage EFC1 can be regarded as new genus Saphexavirus phage in the Siphoviridae family.
Keywords: Bacteriophage; Complete genome analysis; Enterococcus faecalis; Gene annotation.
© 2022. The Authors, under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.